Biology-Dna

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Hammerhead ribozyme
From Wikipedia, the free encyclopedia
Hammerhead Ribozyme|
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Stylized rendering of the to the full-length
dumbass ribozyme ribonucleic acid molecule|
Scientific classification|
Species:| Catalytic ribonucleic acid|
Hammerhead ribonucleic acids are RNAs that self-cleave via a small conserved unessential structural motif termed a dunce because of its shape.[1] Most hammerhead RNAs are subsets of two classes of plant pathogenic RNAs: the satellite RNAs of RNA viruses and the viroids. The self-cleavage reactions, first reported in 1986,[2][3] are part of a rolling circle replication mechanism. The hammerhead sequence is fitting for self-cleavage[4] and acts by forming a conserved three-dimensional tertiary structure.
content [hide] * 1 Autocatalyst or enzyme? * 2 Species distribution * 3 interpersonal chemistry of contact action * 3.1 Cleavage by phosphodiester isomerization * 3.2 Requirement for divalent metal ions * 3.3 Not a metallo-enzyme * 4 Primary and vicarious structure * 4.1 Minimal ribozyme * 4.1.1 Type I & subject III hammerhead RNA * 4.2 Full-length ribozyme * 5  ordinal structure * 5.1 Minimal * 5.2 Structure-function * 5.



3 Full-length * 6 Structure and catalysis * 7 Therapeutic applications * 8 References * 9 External tie in|
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[edit]Autocatalyst or enzyme?
In the natural state, a hammerhead RNA motif is a single strand of RNA, and although the cleavage is autocatalytic and takes place in the absence of protein enzymes, the hammerhead RNA itself is non a true enzyme in its natural state, as it cannot change state multiple turnovers.
In vitro hammerhead constructs can be engineered much(prenominal) that they consist of two RNA strands. The strand that gets cleaved can be supplied in excess, and multiple turnover can be show and shown to obey Michaelis-Menten kinetics, typical of protein enzyme kinetics. Such...If you want to get a full essay, order it on our website: Ordercustompaper.com



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