Biochemistry

Introduction

UV/VIS spectroscopy is an analytical technique in which pop out heartiness from the visible to ultraviolet region is interacted with a judge in order to perform an compendium. As a result, the spectrum obtained is use to gather important information about the render such as its atomic and molecular energy levels as soundly as it chemical bonds and so on. This technique is mostly utilise for qualitative analysis to identify the chemical structure of a given sample, but it is also used for quantitative analysis to measure the amount of material or substance in a sample (Housecroft & Constable, 2006). The principle of UV/VIS spectroscopy involves chemical bonds in which the atomic orbitals of a particular sample molecule merge to form molecular orbitals. These molecular orbitals be occupied with electrons that have various energy levels (Souta et al., 2010). Depending on the oddball of electron bonding the molecular orbitals possess, the electrons can be categorize into three groups; ???single bond),????multiple bond), or non-bonding??When these electrons are bombarded with beams of catch fire energy of a specific wavelength, they get excited and their kinetic energy increase, which cause them to occupy a higher energy level. This leads to the absorption of light energy. There are many uses of UV/VIS spectroscopy.



For instance, it is used to determine food colours in mixtures as well as thermal analysis of DNA strands. some other uses include investigations involving kinetics rate law, the analysis of a vitamin B mixture involving multi-component analysis, and the analysis of water samples containing nitrate (Housecroft & Constable, 2006).

Aim

The get hold of of experiment 1 was to determine the protein concentration of two different samples by constructing a standard calibration curve use standard solutions whereas the aim of experiment 3 was to determine and equation the absorption spectra of deoxy- and oxy-haemoglobin. In addition, experiment 3 was carried...If you want to get a full essay, order it on our website: Ordercustompaper.com



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