Lab Report

Cory Smith

Date performed-14-Nov-2011

Lab session: Tuesday Evenings 6 pm

Lab #18 Extraction of DNA from W catch fire Germ
Purpose
The purpose of this experiment is to call down DNA from wheat germ. The experiment will use heat and enzymes to denature proteins and laundry detergent to open cell and membranes.

Method
ignite to denature proteins:

1. implement a weighing dish and an electronic balance and obtain 0.5g of raw mass wheat germ. ordain that sample into 50ml beaker.
2. Put about 50 ml distilled body of water into a 250 ml beaker and heat until 55 degrees Celsius no more(prenominal) than 60 degrees Celsius. Stir water periodically and monitor temperature.
3. Use 50 ml graduated cylinder to put 10 ml of heated water into beaker containing sample from step 1. birl mixture for three minutes.
4. Add 10-12 drops of liquified dishwashing detergent to the mix in step 3 then twisting for another 5 minutes.
5. Let beaker sit for 1-3 minutes to seize the solid material to settle to bottom, leaving cloudy liquid above it.
6. Using a clean plastic eye eye dropper remove enough of cloudy liquid from the beaker to fill 10 cm test thermionic valve 1/3 full. dip the test tube and use a plastic dropper to remove any foam or bubbles that may induct formed on top of the cloudy liquid.
7.



Put 4-ml of alter inebriant into a 10ml graduated cylinder.
8. Tilt the test tube and slowly pour the alcohol down the sides so the alcohol forms a seam on top of cloudy liquid. Do not mix the layers together.
9. The DNA released from the wheat germ cells is in the lower, water containing cloudy layer. The DNA is not soluble in alcohol. Precipitate more DNA from the water layer by carefully inserting wooden splint through the alcohol layer to bottom of test tube. Pull the wooden splint up to the top and observe the DNA precipitate.

Effects of detergent on cell membranes:

1. Follow the steps of Part A, except do not do step 4. Record results in display board and be sure to note differences....If you want to get a full essay, order it on our website: Ordercustompaper.com



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